Using individual differences constructs, the current study used cross-sectional data to examine the mediating role of negative self-statements during public speaking on the relationship between fear of negative evaluation and public speaking anxiety (a type of performance anxiety), and how this relationship was moderated by positive self-statements during public performance. The sample comprised 319 adults (men = 105, women = 214) from the general Australian community, with ages ranging from 18 years to 65 years. All participants completed questionnaires covering the different study variables. The findings showed that there was partial mediation by negative self-statements on the relationship between fear of negative evaluation and performance anxiety. There were also moderation effects by positive self-statements for this relationship. Additionally, moderation by positive self-statements was evident at all levels of positive self-statements. The theoretical and clinical implications of the findings for public speaking anxiety are discussed.