Previous research has shown increased mindfulness to be associated with enhanced subjective well-being and self-acceptance. Improvements in self-acceptance, in turn, predict positive outcomes in psychotherapy across a wide range of problems and populations. Peace of mind (PoM) represents a special case of subjective well-being that is particularly important in a traditional Chinese cultural context. This study explored the mediating influences of self-acceptance on the relationship between mindfulness and peace of mind. Two hundred and twelve participants completed the Five Facet Mindfulness Questionnaire, Peace of Mind Scale, and Self-acceptance Questionnaire. Results showed that mindfulness was significantly positively correlated with peace of mind, and the positive association between mindfulness and peace of mind was mediated by self-acceptance. Limitations, clinical implications, and directions for future research are discussed.