The present study aimed to investigate the psychometric properties of the Freiburg Mindfulness Inventory (FMI) in a nonclinical Chinese college student sample. Effective data were collected from 718 students of four distinct universities in north, northwest, southeast, northeast China, and 124 of them were retested after 2 weeks. The Chinese version of Freiburg Mindfulness Inventory (FMI-13) had acceptable internal consistency and test–retest reliability. Confirmatory factor analysis showed that the two-factor solution was better than one-factor solution. The scores of revised FMI-13 had significant negative correlation with the trait of anxiety and depression, and positive correlation with self-esteem, and relations between mindfulness and anxiety, depression, and self-esteem were more due to the acceptance factor than the presence factor. The Chinese version of FMI-13 has acceptable psychometric quality and it can be used to measure mindfulness in Chinese population.